A new bamboo-feeding species of the genus Mukaria Distant (Hemiptera:Cicadellidae:Deltocephalinae:Mukariini), M. hainanensis sp. nov., is described and illustrated. The new species was collected on bamboo (Bambusa chungii) from Jiangfengling National Natural Reserve, Hainan Province, China. The new species is closely related to M. ablinotata Cai & Ge, 1996, but can be distinguished by the characters below: forewing yellowish brown; ventral process of male pygofer long and sinuate, turning outside apically at ventral view; male aedeagus without process between two shafts, inside process of shaft near apex stout and straight. In this paper, the external morphological characteristics of the new species are described in detail. A key to Chinese species of the genus Mukaria is compiled, and the photograph illustrations of the new species and M. ablinotata, colour photographs of new species and its host plants are provided. The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University, Guiyang, China.