For a long time it was considered, that Diaporthe helianthi is the only species of the genus Diaporthe, as a causal agent of Phomopsis stem canker, which is found everywhere in the sunflower fields. In Russia this species is included in the list of quarantine objects, which have limited distribution in the country. However, these data about distribution are based on the identification of the pathogen solely by symptoms or morphological features formed by isolates in the pure culture. Currently it is accepted by mycologists that the correct identification of species of the genus Diaporthe should be performed by molecular genetic features. To date there are at least 13 species of this genus, associated with sunflower. A comprehensive analysis of biodiversity and geographic distribution of Diaporthe species in Russia has not been performed. The aim of this work was identification of five Russian Diaporthe isolates from sunflower. According to both molecular-phylogenetic and morphology data, studied isolates were identified as D. gulyae, D. phaseolorum, and D. eres. To our knowledge, these findings are the first reports of these species in Russia. Currently, detection of D. helianthi on sunflower in Russia was not supported by molecular studies.