Apple of Peru (Nicandra physalodes) is a member of the Solanaceae family and one of the serious weed threats in crop fields. It is tolerant to commonly used herbicides and competes with cultivated crops. Additionally, apple of Peru and other solanaceous weeds are known as an alternative host of several viruses. Pepper mottle virus (PepMoV) in the genus Potyvirus (family Potyviridae) is a monopartite, positive-sensed, single-stranded RNA virus. PepMoV causes economic losses to peppers and tomatoes in Honduras, South Korea, and Hawaii. In February 2018, apple of Peru plants were observed with severe virus-like symptoms: mosaic, mottling, and crinkling of leaves in a fallow area at the University of Hawaii in Manoa. Based on the results of ELISA tests, polymerase chain reaction assays and BLAST analysis of the nucleotide sequences, the causal agent was identified as PepMoV. This is thought to be the first report of apple of Peru as a host of PepMoV in Hawaii.