Longidorus pinus sp. n. from China is characterised by females 3.3-4.9 mm long; lip region 8-12 µm diam., distinctly offset by a constriction; amphidial fovea pocket-shaped without basal lobes; a short odontostyle (67-81 µm); anteriorly located guide ring (30-38 µm); and short and conoid tail (31-40 µm long, c=84-138, c′=1.2-1.9), with one or two pairs of caudal pores on each side. Males were not found. Three developmental juvenile stages were identified, the first-stage juvenile with conoid tail (c′=2.4-2.9). The polytomous key codes for the new species are: A2(3), B1, C23, D4, E1, F2, G23, H23, I1, J2, K6. Molecular phylogenetic analyses based on SSU, D2-D3 of LSU, ITS1 and ITS2 rDNA sequences indicate that L. pinus sp. n. is closely related to L. hangzhouensis, L. asiaticus, and L. camelliae, all of which apparently share a common Asiatic geographic origin. In addition, a key for identification of Longidorus found in China, based on morphological characters of both female and first-stage juvenile, is provided.