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Identification of Tomato yellow leaf curl virus-IR and associated Tomato leaf curl betasatellite infecting common bean (Phaseolus vulgaris) in Oman.

Shahid, M. S. ; Al-Shihi, A. A. ; Al-Sadi, A. M. ; Briddon, R. W.

Plant Disease 2018 Vol 102 No. 9 pp. 1864;


In 2016, foliar yellow mosaic, severe curling, and crumpling symptoms, with an incidence of 20 to 35%, were observed on bean (P. vulgaris) plants in mixed crop (tomato, chili and watermelon) fields in the Al-Batina region of Oman together with a high-density population (>10 adults/leaf) of Bemisia tabaci (MEAMI). Five bean plants with prominent symptoms and two nonsymptomatic plants were collected from two farms ∼5 km apart. An initial analysis of crude sap extracted from symptomatic leaf samples under electron microscopy with negative staining showed the presence of twinned-icosahedral particles (18 to 20 nm) typical of geminiviruses. PCR assay and rolling-circle amplification were carried out. BLAST comparison of the sequence to sequences available in the GenBank nucleotide sequence database showed 98% nt identity to an isolate of the "Iran" strain of Tomato yellow leaf curl virus (TYLCV; FJ956703) previously identified infecting tomato in Oman. PCR amplification using universal betasatellite primers showed the presence of a betasatellite in all five plants. For one sample, the PCR product was cloned and sequenced. The amplicon contained 1 366 nt and showed an arrangement typical of betasatellites, with a single conserved gene (βC1) in the complementary-sense. Sequence comparisons showed the sequence to have 99% nt identity to an isolate of tomato leaf curl betasatellite (ToLCB) previously identified in Oman. This is thought to be the first record of TYLCV-Iran, in conjunction with ToLCB, infecting P. vulgaris in Oman.