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First report of Tomato leaf curl New Delhi virus infecting zucchini in Morocco.

Sifres, A. ; Sáez, C. ; Ferriol, M. ; Selmani, E. A. ; Riado, J. ; Picó, B. ; López, C.

Plant Disease 2018 Vol 102 No. 5 pp. 1045;


During spring 2017, generalized leaf symptoms including yellowing and curling, as well as stunting of plants, were observed in several zucchini (Cucurbita pepo) fields of one of the most common commercial Moroccan cultivars, Suha F1 (Sakata Vegetables Europe S.A.S.) in the region of Agadir, Morocco. Leaf samples from nine symptomatic and three asymptomatic plants were first tested for Tomato leaf curl New Delhi virus (ToLCNDV) infection with an ImmunoStrip kit. Only the symptomatic samples were positive for ToLCNDV infection. To confirm the identity of the virus, DNA was isolated from the symptomatic leaf samples and analysed by PCR. Amplicons were obtained from all the samples that had tested positive for ToLCNDV with the ImmunoStrip kit. PCR products were directly sequenced and BLAST analysis showed nucleotide identity higher than 99% with sequences from ToLCNDV isolates of Spain. This is thought to be the first report of the presence of ToLCNDV in Morocco.