In the summers of 2015 and 2016, a series of field surveys were conducted in Shandong Province of China for virus infections in the two major sesame cultivars in the province, Zhongzhi 11 and 13. Approximately 20 to 30% of plants showed mosaic, leaf puckering, and internode shortening, and 68 samples were collected from both symptomatic and asymptomatic plants. Eight symptomatic plants (three from Ji'ning in 2015 and five from Ji'nan in 2016) showed positive reactions to Tobacco mosaic virus (TMV) in double antibody sandwich ELISA tests. This result was further confirmed by reverse-transcription PCR and nucleotide sequence analysis. TMV has been reported to infect more than 300 species of 30 families including Solanaceae, Amaranthaceae, Chenopodiaceae, Cucurbitaceae, and Cruciferae. Isolates Ji'yang1 and Ji'ning were obtained by single lesion inoculation in Chenopodium amaranticolor [C. giganteum] and were inoculated onto Nicotiana tabacum cultivar NC89 and sesame. Both of these plants showed symptoms of mosaic. This is thought to be the first report of the natural TMV infection of sesame.