Cocksfoot mottle virus (CfMV) was detected for the first time in Ohio, USA, in samples collected from March to April 2016. Symptomatic plants (15 to 30) expressing virus-like symptoms, including stunting, chlorosis, and mosaic, were collected from soft red winter wheat production fields and nearby volunteer wheat at 40 locations across 20 counties. The identity of the virus was confirmed by RNA sequencing, reverse transcription PCR assay and nucleotide sequence analysis. This is thought to be the first report of a natural infection of CfMV in wheat. Additionaly, this is only the second report of this virus in the USA and is geographically distant from the first report in Oregon.