Objectives: To determine the biotypes of Bemisia tabaci (Gennadius) found in the Tibet Autonomous Region, China. Methods: Morphological characteristics of whiteflies were observed under a 3D digital microscope, and the biotype of whiteflies collected from Lhasa, Tibet, identified on the basis of variation in the molecular marker mtCO I. Results: Analysis of variation in the mtCO I molecular marker shows that whiteflies collected in Tibet were of the Q biotype. Conclusion: The results of both morphological identification and molecular analysis show that the whiteflies collected in Lhasa, Tibet Autonomous Region, were of the Bemisia tabaci Q biotype. This is the first report of the Bemisia tabaci Q biotype from the Tibet Autonomous Region.