In May 2013, dry, round, sunken zones (10-20 mm) were observed on pepper fruits in the Cameron Highlands, Pahang, Malaysia. Eventually, the lesions turned brown, coalesced and the fruits mummified on the plants. Disease incidence was up to 60% in severely infected pepper glasshouses. Diseased samples were collected randomly from different infected glasshouses. The causal pathogen was identified as Colletotrichum nymphaeae based on morphological, genetic (ITS and PCR) and pathogenicity analyses. This is thought to be the first occurrence of anthracnose fruit rot caused by C. nymphaeae on pepper in Malaysia and worldwide.