In 2013, leaves from tamarillo (Solanum betaceum [Cyphomandra betacea]) plants showing severe virus-like symptoms (mosaic, mottling, and leaf deformation) were collected from orchards in Pichincha and Tungurahua (Ecuador) and pooled into 20-g batches. Double-stranded RNA (dsRNA) was extracted from three independent batches and used to generate cDNA libraries to screen for RNA viruses by shotgun cloning. Sequencing of 400 transformed plasmids revealed the presence of the potyviruses Potato virus Y (PVY), Potato virus V (PVV) and Peru tomato mosaic virus (PTV), and the polerovirus Potato leafroll virus (PLRV). To determine the occurrence of the viruses, 200 leaf samples from symptomatic and asymptomatic tamarillo plants were collected from commercial orchards in Pichincha and Tungurahua and tested by RT-PCR. For symptomatic plants, PVY and PLRV were the most common with 64% and 30%, respectively. PVV and PTV were found in 8% and 10%, respectively, with only 4% of the plants coinfected by both viruses. Plants coinfected by PVY and PLRV were the most common with 22%, followed by plants coinfected by PVY and PTV (10%). Asymptomatic plants did not test positive for any of the viruses. This paper reports for the first time in tamarillo the presence of PVV and PTV, two potyviruses once considered strains of PVY.