First report of sclerotinia fruit rot of Citrus paradisi caused by Sclerotinia sclerotiorum in Pakistan.
Hanif, S. ;
Hafeez, R. ;
Akram, W. ;
Ashfaq, M. ;
Grapefruit (Citrus × paradisi) has become a crop of interest in Pakistan. In November 2014, while monitoring postharvest decay of fruits during storage in Lahore, symptoms of soft watery rot with white fuzzy mycelial growth were observed on approximately 40% of 'Duncan' grapefruit. Dark lesions on the fruit surface containing black sclerotia were also observed. Necrotic tissues from the margin of the diseased portion of the fruit were aseptically removed and placed on potato dextrose agar plates containing 150 µg ml/l streptomycin sulfate. Based on morphological characteristics, sequence analysis of the rDNA internal transcribed spacer region and pathogenicity tests, the causal agent was identified as Sclerotinia sclerotiorum. This is thought to be the first report of postharvest fruit rot of grapefruit caused by S. sclerotiorum in Pakistan.