The study was conducted on a common complaint of strawberry farmers regarding stunted growth and death of strawberry runner saplings. Of the 14 symptomatic samples, 9 were recovered with Fusarium oxysporum isolates, 03 isolates of Pythium aphanidermatum (Edson) Fitzp., and 02 isolates of Fusarium solani (Mart.) Sacc., from the diseased strawberry roots on mycological media. The pathogenicity test confirmed F. oxysporum isolates as highly virulent in screen house conditions. The infected plant growth was retorted and finally died ironic to control treatments. The strains of F. Solani and P. aphanidermatum showed a-pathogenic effect on the host when bio assayed @ 1.0×107 spores added to the plant soil. The F. oxysporum strains were treated in vitro with fungicide concentrations. The fungicide Carbendazim showed the lowest LC50 value (4.5 cm diameter radius inhibition zone) 0.032 to 0.036 ppm and Difenoconazole showed 0.04 to 0.052 ppm against the spores germination on poised mycological medium. Carbendazim and Difenoconazole were also efficient to restrict mycelia growth on the poised mycological media with LC50 ranges from 5.4-7.9 and 5.7-13.8 ppm, respectively.