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Title Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain.

Authors Ambrós, S.; Martínez, F.; Ivars, P.; Hernández, C.; Iglesia, F. de la; Elena, S. F.

Author Affiliation Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, 46022 Valencia, Spain.

Author Email sambros@ibmcp.upv.es

Journal European Journal of Plant Pathology 2017 Vol 149 No. 2 pp. 261-268

ISSN 0929-1873


Abstract

Tomato is known to be a natural and experimental reservoir host for many plant viruses. In the last few years a new tobamovirus species, Tomato mottle mosaic virus (ToMMV), has been described infecting tomato and pepper plants in several countries worldwide. Upon observation of symptoms in tomato plants growing in a greenhouse in Valencia, Spain, we aimed to ascertain the etiology of the disease. Using standard molecular techniques, we first detected a positive sense single-stranded RNA virus as the probable causal agent. Next, we amplified and sequenced its full-length genomic RNA which identified the virus as a new ToMMV isolate. Through extensive assays on distinct plant species, we investigated the host range of the Spanish ToMMV isolate. Several plant species were locally and/or systemically infected by the virus, some of which had not been previously reported as ToMMV hosts despite they are commonly used in research greenhouses. Finally, two reliable molecular diagnostic techniques were developed and used to assess the presence of ToMMV. This is the first observation of ToMMV in tomato plants in Europe. We discuss the possibility that, given the high sequence homology between ToMMV and Tomato mosaic virus, the former may have been mistakenly diagnosed as the latter by serological methods.
Publication type
Journal article

Publisher
Springer

Year of publication
2017

Location of publication
Dordrecht

Country of publication
Netherlands

Language of publication
English

Language of summary
English

Geographical location
Europe
Spain


Record number
20173321819

Organism descriptor
Arabidopsis thaliana
Capsicum annuum
Chenopodium quinoa
Nicotiana benthamiana
Solanum lycopersicum
Tobamovirus
Virgaviridae
viruses


Descriptor
geographical distribution
host range
new geographic records
nucleotide sequences
pathogens
plant pathogens
plant viruses
RNA
techniques
tomatoes


Identifier
DNA sequences
Lycopersicon esculentum
phytopathogens
quinoa
ribonucleic acid
Tomato mottle mosaic virus
viruses of plants


Broad Term
Arabidopsis
Brassicaceae
Brassicales
eudicots
angiosperms
Spermatophyta
plants
eukaryotes
Capsicum
Solanaceae
Solanales
Chenopodium
Chenopodiaceae
Caryophyllales
Nicotiana
Solanum
Developed Countries
European Union Countries
Mediterranean Region
OECD Countries
Southern Europe
Europe
Virgaviridae
positive-sense ssRNA Viruses
ssRNA Viruses
RNA Viruses
viruses