On 17 April 2010, twelve plants of S. viarum located close to a S. betaceum crop (tree tomato) in the province of Caldas (Department of Antioquia, central northwestern Colombia) showed symptoms similar to late blight caused by P. infestans on S. tuberosum (potato). Microscopic characteristics were consistent with Phytophthora species. DNA extraction was performed from the P. infestans isolate. Four nuclear loci (internal transcribed spacer, β-tubulin, Ras and Avr3a) and one mitochondrial gene (cytochrome c oxidase 1; Cox1) were amplified and sequenced. Sequences were compared with GenBank databases using Blastn. In all cases, the best hits corresponded to P. infestans (GenBank accession no. HQ639930 for Avr3A, HQ639931 for β-tubulin, HQ639932 for Cox1, HQ639933 for iRas, HQ639934 for Ras, and JF419363 for ITS). This is thought to be the first report of P. infestans isolated from S. viarum in Colombia.