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Title First report of grapevine rupestris vein feathering virus in grapevine in Slovakia.

Authors Glasa, M.; Predajňa, L.; Wetzel, T.; Šoltys, K.; Sabanadzovic, S.

Author Affiliation Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, 84505 Bratislava, Slovakia.

Author Email virumig@savba.sk

Journal Plant Disease 2019 Vol 103 No. 1 pp. 170

ISSN 0191-2917


Abstract

Grapevine rupestris vein feathering virus (GRVFV), a tentative member of the genus Marafivirus (family Tymoviridae), was originally reported from a Greek source of Vitis vinifera 'Sultanina' displaying asteroid-like symptoms and eliciting vein feathering symptoms upon grafting on Vitis rupestris. In August 2017, samples consisting of main veins of fully developed leaves of two grapevine plants were used for total RNA isolation. Ribosome-depleted RNA preparations were used for cDNA library synthesis followed by high-throughput sequencing (HTS) on an Illumina MiSeq platform (300-bp paired-end sequencing). BLAST analyses of contigs assembled from HTS reads from these grapevine samples, referred to as SK809 and SK933, indicated the presence of multiple virus infections, including GRVFV (a total of 137 and 288 reads, respectively), mapped to the reference GRVFV genome. The contigs from SK809 (530 bp) and SK933 (1 959 bp) showed 82 and 85% identity, respectively, to GRVFV. BLAST analyses revealed the additional presence of Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine virus T in SK809 and GLRaV-2, GLRaV-3, Grapevine rupestris stem pitting-associated virus, Grapevine Syrah virus 1, and Hop stunt viroid in SK933. A pool of grapevine samples from four localities in western Slovakia was screened by reverse transcription polymerase chain reaction to confirm the presence of GRVFV. Phylogenetic analysis of all corresponding available partial sequences in GenBank and 14 Slovak isolates suggested the existence of two molecular groups. These results indicate multiple introductions of GRVFV in the country. This is thought to be the first report of GRVFV infecting grapevines in Slovakia.
Publication type
Journal article

Publisher
American Phytopathological Society (APS Press)

Year of publication
2019

Location of publication
St. Paul

Country of publication
USA

Language of publication
English

Language of summary
English

Geographical location
Slovakia

Record number
20193045863

Organism descriptor
Ampelovirus
Betaflexiviridae
Closteroviridae
Foveavirus
Grapevine leafroll-associated virus 1
Grapevine rupestris stem pitting-associated virus
Grapevine Syrah virus 1
Hop stunt viroid
Marafivirus
Tymoviridae
viruses
Vitis
Vitis rupestris
Vitis vinifera


Descriptor
geographical distribution
grapes
mixed infections
new geographic records
pathogens
plant diseases
plant pathogens
plant viruses


Identifier
Grapevine rupestris vein feathering virus
grapevine virus T
multiple infections
phytopathogens
viruses of plants


Broad Term
Closteroviridae
positive-sense ssRNA Viruses
ssRNA Viruses
RNA Viruses
viruses
Tymovirales
Betaflexiviridae
Ampelovirus
Foveavirus
Marafivirus
Tymoviridae
Hostuviroid
Pospiviroidae
viroids
Central Europe
Europe
Developed Countries
European Union Countries
OECD Countries
Vitis
Vitaceae
Vitales
eudicots
angiosperms
Spermatophyta
plants
eukaryotes