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Title A novel strain of pepper leafroll virus infecting common bean and soybean in Ecuador.

Authors Fiallo-Olivé, E.; Chirinos, D. T.; Castro, R.; Navas-Castillo, J.

Author Affiliation Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Consejo Superior de Investigaciones Científicas - Universidad de Málaga (IHSM-CSIC-UMA), 29750 Algarrobo-Costa, Málaga, Spain.

Author Email efiallo@eelm.csic.es

Journal Plant Disease 2019 Vol 103 No. 1 pp. 167-168

ISSN 0191-2917


Abstract

Symptoms resembling those caused by begomoviruses have been observed in common bean and other leguminous crops in Ecuador in the last few years. The only begomovirus species found infecting legumes (common bean and cowpea) in the country to date is Cabbage leaf curl virus. During May to June 2017, a survey of leguminous plants was conducted in the Ecuadorian localities of Santa Elena (Santa Elena province), Milagro (Guayas province), and Pallatanga (Chimborazo province). Twenty-four plants showing leaf deformation and/or mosaic symptoms were sampled, including common bean (Phaseolus vulgaris, n=21), soyabean (Glycine max, n=2), and pigeon pea (Cajanus cajan, n=1). Most common bean plants were infested with whiteflies (Aleyrodidae) that were also collected. Based on the results of polymerase chain reaction assay, rolling-circle amplification and sequence comparison, the causal agent was identified as Pepper leafroll virus (PepLRV). Based on the species/strain demarcation threshold for the viruses in the genus Begomovirus, the isolate characterized belongs to a new strain of PepLRV. DNA sequence analysis identified the whiteflies as Bemisia tabaci Middle East-Asia Minor 1 (MEAM1, formerly B biotype), which is known to be an efficient vector of begomoviruses. This is thought to be the first report of PepLRV in Ecuador and of soyabean as a host for the virus, which had been identified only in Peru to date. Also, this is thought to be the first molecular evidence of the presence of B. tabaci MEAM1 in the country.
Publication type
Journal article

Publisher
American Phytopathological Society (APS Press)

Year of publication
2019

Location of publication
St. Paul

Country of publication
USA

Language of publication
English

Language of summary
English

Geographical location
Ecuador

Record number
20193045858

Organism descriptor
Begomovirus
Bemisia tabaci
Bemisia tabaci (B biotype)
Cajanus cajan
Geminiviridae
Glycine (Fabaceae)
Glycine max
insects
Phaseolus vulgaris
viruses


Descriptor
arthropod pests
disease vectors
DNA sequencing
geographical distribution
hosts
insect pests
new geographic records
new host records
nucleotide sequences
pathogens
pests
pigeon peas
plant diseases
plant pathogens
plant pests
plant viruses
polymerase chain reaction
soyabeans
symptoms
vectors


Identifier
Bemisia tabaci (MEAM1)
cotton whitefly
DNA sequences
nucleotide sequence analysis
nucleotide sequencing
PCR
Pepper leafroll virus
pest arthropods
pest insects
phytopathogens
soybeans
tobacco whitefly
viruses of plants


Broad Term
Geminiviridae
ssDNA Viruses
DNA Viruses
viruses
Bemisia
Aleyrodidae
Aleyrodoidea
Sternorrhyncha
Hemiptera
insects
Hexapoda
arthropods
invertebrates
animals
eukaryotes
Cajanus
Papilionoideae
Fabaceae
Fabales
eudicots
angiosperms
Spermatophyta
plants
Andean Group
Developing Countries
Latin America
America
South America
Threshold Countries
Glycine (Fabaceae)
Phaseolus