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Title First report of Moroccan pepper virus on clematis in Russia and worldwide.

Authors Zakubanskiy, A. V.; Mitrofanova, I. V.; Chirkov, S. N.

Author Affiliation Lomonosov Moscow State University, Moscow, 119234, Russia.

Author Email s-chirkov1@yandex.ru

Journal Plant Disease 2018 Vol 102 No. 7 pp. 1469-1470

ISSN 0191-2917


Abstract

Clematis (Clematis spp., family Ranunculaceae) is a perennial climbing plant widely used in landscape design. Over 120 local and introduced clematis cultivars are maintained in the field collection of the Nikita Botanical Gardens, Yalta, Russia. Plants displayed leaf chlorosis, mottling, mosaic, and vein yellowing. Next generation sequence analysis of 8 individual or bulked samples representing 20 cultivars, one plant of each selected cultivar, was performed regardless of symptoms. From a single plant of cultivar Valge Daam, 2 500 reads from a total of ∼59 million reads mapped to plant virus sequences related to Moroccan pepper virus (MPV, genus Tombusvirus). Reverse transcription-PCR using total RNA extracted from the presumably infected plant and four MPV-specific primer pairs confirmed the presence of the virus. Using the NGS data, the full-size genome of the Russian MPV isolate (named MPV-Cl) was assembled and the sequence has been deposited in GenBank (MG250195). Phylogenetic analysis of the tombusvirus complete genomes assigned MPV-Cl to a separate cluster consisting of the MPV isolates with 100% bootstrap support. To date, MPV has been reported to infect pepper, tomato, pelargonium, eggplant, escarole, lisianthus, pear, and lettuce, and can persist in soil water and plant debris. This is thought to be the first report of MPV infecting clematis in Russia and worldwide.
Publication type
Journal article

Publisher
American Phytopathological Society (APS Press)

Year of publication
2018

Location of publication
St. Paul

Country of publication
USA

Language of publication
English

Language of summary
English

Geographical location
Russia

Record number
20183233412

Organism descriptor
Clematis
Moroccan pepper virus
Tombusviridae
Tombusvirus
viruses


Descriptor
cultivars
genomics
geographical distribution
hosts
new geographic records
new host records
nucleotide sequences
pathogens
phylogenetics
plant diseases
plant pathogens
plant viruses
polymerase chain reaction
symptoms
world


Identifier
cultivated varieties
DNA sequences
PCR
phytopathogens
Russian Federation
viruses of plants
worldwide


Broad Term
Ranunculaceae
Ranunculales
eudicots
angiosperms
Spermatophyta
plants
eukaryotes
Tombusvirus
Tombusviridae
positive-sense ssRNA Viruses
ssRNA Viruses
RNA Viruses
viruses
APEC countries
Developed Countries